Part:BBa_K4767005

PluxⅠ-RBS-luxR(△2-162)-RBS-gfp-TT

Uses a factor P_luxⅠ(BBa_R0062) ,strong RBS(BBa_J34801), luxR(△2-162)(BBa_K4767000) and gfp coding region(BBa_E0040)and TT(BBa_B0015). This part is an easy BioBrick.

Usage and Biology

This is the response part of the luxR amplifier with the gfp gene as the reporter. In our design, if luxR(△2-162) was expressed as a result of responding, this part can be activated to express gfp and luxR(△2-162), and luxR(△2-162) will functions in a positive feedback loop as it can bind to the P_luxI promoter and activate its own transcription.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1021
  

References

Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1.